Creatine amidinohydrolase

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August undefined, 2024

WebMay 2, 2010 · Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the … WebThis enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. The systematic name of this …

RCSB PDB - 1KP0: The Crystal Structure Analysis of Creatine ...

WebMay 24, 2001 · Creatine amidinohydrolase is an enzyme which has a catalytic action of hydrolyzing creatine to generate sarcosine and urea. Creatine amidinohydrolase can be used for measuring the amount of creatine existing in human serum or urine, and so this enzyme can be used as a diagnostic agent for various diseases including renal diseases. Web中文名:肌酸酶,英文名:CREATINE AMIDINOHYDROLASE,CAS:37340-58-2,概述人类血清中的肌酸酐浓度，是反应肾脏功能的重要指标之一。人们提出了用酶法代替化学测定。由于酶促反应专一性强，能大大提高测定结果的可靠性，是肌酸酐测定的发展方向。酶促检测方法的原理是，利用肌酸酐酶(Creatininase，EC 3.5.2.10 ... flood insurance destin fl

A stable three enzyme creatinine biosensor. 2. Analysis of the …

WebCreatine Amidinohydrolase catalyzes the hydrolytic reaction converting creatine to sarcosine and urea. The enzyme is purified from a microorganism. The molecular weight … WebCreatinine Amidohydrolase (from Pseudomonas sp.) Enzymes This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, … WebApr 1, 2005 · Creatinase (creatine amidinohydrolase, EC 3.5.3.3) from Pseudomonas putida is a homodimer of 45 kDa subunit molecular mass, the three-dimensional structure of which is known at 1.9 A resolution. flood insurance credits

Creatinine hydrolase and creatine amidinohydrolase: I.

WebENZYME entry: EC 3.5.3.3. creatinase. creatine amidinohydrolase. All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in different formats, align etc. All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.5.3.-. All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.5.-.-. WebThe creatine is converted to sarcosine and urea by creatine amidinohydrolase. The sarcosine, in the presence of sarcosine oxidase, is oxidized to glycine, formaldehyde, … great member service imagesCreatinase is used as a key enzyme for enzymatic measurement of creatinine which catalyzes the hydrolysis of creatine to sarcosine and urea. It has been found in various kinds of bacteria such as Flavobacterium, Pseudomonas, Arthrobacter and Bacillus [6, 28, 30,31,32]. In this work, we analyzed the … See more In order to provide an initial characterization of the optimal temperature for wild-type afCR (WT), we first examined its activity across temperatures ranging … See more Based on the report of a I304L/F395V double mutant variant of creatinase from Erwinia with lower KM than its wild type enzyme [38], we first … See more To assess the kinetic stability of the variants, activity was measured across a range of temperatures (35–70 °C) to determine the temperatures at which enzyme activity was … See more great memes for a great wife

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Creatine amidinohydrolase

WebThe enzyme creatine amidinohydrolase is a clinically important enzyme used in the determination of creatinine in blood and urine. Continuous use biosensors are becoming … WebOct 2, 2024 · Creatine amidinohydrolase. E.C.3.5.3.3. Hydrolyzes creatine to sarcosine and urea. Links ? Statistics ? Structure ? Conserved Features/Sites? PubMed …

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WebApr 1, 2009 · Creatine amidinohydrolase (creatinase, EC 3.5.3.3) is an important enzyme for creatinine concentration determination in biological fluids to evaluate renal damage. … WebThe enzymatic assay for creatinine involves a series of coupled enzymatic reactions including creatininase enzymatic conversion of creatinine into the product creatine …

WebThe creatine is converted to sarcosine and urea by creatine amidinohydrolase. The sarcosine, in the presence of sarcosine oxidase, is oxidized to glycine, formaldehyde, … WebThe enzymatic assay for creatinine involves a series of coupled enzymatic reactions including creatininase enzymatic conversion of creatinine into the product creatine which itself is converted to sarcosine by creatine amidinohydrolase (creatinase), followed by oxidation of sarcosine by sarcosine oxidase (SOD) producing hydrogen peroxide.

WebJul 1, 1982 · Abstract. A new enzymatic method is described for the determination of creatine in serum and urine using creatine amidinohydrolase (EC 3.5.3.3), sarcosine oxidase (EC 1.5.99.1) and formaldehyde dehydrogenase (EC 1.2.1.1). The principle of the method is as follows. Creatine is degraded to sarcosine and urea, and the sarcosine … WebTo create a potentiometric creatinine sensor, creatinine iminohydrolase, which catalyzes the production of ammonia from creatinine, can be immobilized on the surface of an …

WebOct 1, 1979 · Abstract. Creatinine amidohydrolase [EC 3.5.2.-; creatininase] was purified in an overall yield of 11% from cell-free extract of Pseudomonas putida var. naraensis, …

WebThe systematic name of this enzyme class is creatine amidinohydrolase. This enzyme participates in arginine and proline metabolism. Form. Liquid or lyophilized powder. Enzyme Commission Number. EC 3.5.3.3. CAS No. 37340-58-2. Storage. Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC. great memes 2021WebDescription Creatine Amidinohydrolase catalyzes the hydrolytic reaction converting creatine to sarcosine and urea. The enzyme is purified from a microorganism. The molecular weight of the enzyme is approximately 67,000. great member service quotesWebMar 15, 2024 · Name:CREATINE AMIDINOHYDROLASE,CAS:37340-58-2.Use:The concentration of creatinine in human serum is one of the important indicators of renal function. Enzymatic methods have been proposed in place of chemical assays. Because of the high specificity of enzymatic reaction, it can greatly improve the reliability of the … flood insurance detached structuresWebcreatinine amidohydrolase and creatine amidinohydrolase are prepared by a process comprising culturing (a) alcaligence spec. ws 51400 or (b) penicillium ws 90001; in a creatinine-containing medium, digesting same, obtaining the creatinine amidiodohydrolase in substantially pure form from the water-soluble digestion fraction and, optionally … flood insurance detached structure ruleWebCreatine Phosphate, Disodium Salt, CAS 19333-65-4, is a high-energy phosphate compound that stores energy from mitochondria whenever sufficient amounts of ATP are … flood insurance detached structure exemptionWebCreatine amidinohydrolase (EC 3.5.3.3, from Microorganism) Creatine + H2O ⇒ Sarcosine + Urea. Specification. PREPARATION and SPECIFICATION. Specification. … flood insurance diagram 6WebCreatine Amidinohydrolase CAS Number: 37340-58-2 Enzyme Commission number: 3.5.3.3 ( BRENDA, IUBMB) EC Number: 253-471-1 MDL number: MFCD00130875 NACRES: NA.54 Pricing and availability is not currently available. Properties biological source bacterial (Actinobacillus spp.) Quality Level 200 form lyophilized powder specific … great memories nursery