WebJul 1, 2005 · Input options for E-RNAi. Shown here is the input form of E-RNAi. The user is asked to choose a run option ( de novo design, probe retrieval or probe evaluation) and to identify the organism for which to predict probe specificity. Users can also set the number of designed primers to be evaluated and of final results shown in the output. WebJul 8, 2024 · To determine the effect of LmapoLp-II/I RNAi on lipid synthesis, the expression of lipid synthesis related genes, including acetyl-CoA Carboxylase ( LmACC ), fatty acid synthases ( LmFAS1, LmFAS2, and LmFAS3 ), fatty acid elongases ( LmELO1-7 ), and Cytochrome P450s ( LmCYP4G62 and LmCYP4G102) identified in our lab, was …
Trafficking Along the Secretory Pathway in Drosophila Cell …
WebE-RNAi can retrieve sequence from the Generic Genome Browser (GBrowse) now. Just select your organism of interest from below and submit using the GBrowse button. Boutros lab, E-RNAi-Version 3.2 For suggestions please contact us at [email protected]. WebSep 24, 2012 · Genome-scale RNA-interference (RNAi) screens are becoming ever more common gene discovery tools. However, whilst every screen identifies interacting genes, … hdb jurong west branch address
Long dsRNA / siRNA design options in E-RNAi version 3
WebApr 27, 2024 · RNA interference (RNAi) primers were designed using the E-RNAi webservice 1. The T7 promoter sequence (5′-TAATACGACTCACTATAGGG-3′) was incorporated into all forward and reverse RNAi primers. The target gene fragment was amplified using Ex Taq DNA Polymerase (Takara, Kusatsu-shi, Japan). WebOct 12, 2024 · RNA interference (RNAi) is a valuable research tool for probing gene function and has already been widely applied to P450 function analysis and honey bee biology research (Li-Byarlay et al. 2013; Li et al. 2016 ). Weblong dsRNAs (E-RNAi Webservice, German Cancer Research Center): T7-parkin forward primer, 5-taa tac gac tca cta tag ggC TGT TGA CAC GCG AGG AGT A-3, and T7-parkin reverse primer, 5-taa tac gac tca cta tag ggA TTT TGG ACA GGG CTT TGT G-3; T7-PINK1 forward primer, 5- taa tac hdb key collection appointment